Advantage Of The Gene Gun

The advantage of gene gun in cotton transgenic

In 1994, Ye Wuwei and others of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences developed a rapid method for the rapid transformation of cotton gene guns. Through the third generation of portable gene guns and optimized transformation parameters, micro-injury to flower buds and pollen was formed during the transformation of living organisms. , greatly shortening the cycle of obtaining transgenic plants.

The method comprises the following steps:

(1) using a gene gun bombardment method, bombarding a vector containing a foreign gene into the pollen of the male parent cotton;

(2) Pollinating the paternal pollen after bombardment with the gene gun into the living mother cotton;

(3) The seeds of the mother cotton after pollination are the seeds of genetically modified cotton, which realizes the genetic transformation of cotton. The method does not require a tissue culture step, and realizes gene conversion directly on the living cotton, which is practical, easy to operate, independent of tissue culture, and has good stability.