Scientz08-III Non-touch Ultrasonic Homogenizer In Chromatin Immunoprecipitation Sample Preparation

scientz08-III non-touch ultrasonic homogenizer in chromatin immunoprecipitation sample preparation

Test result (Figure 1): ① scientz 08-III can effectively interrupt chromatin under a shorter total ultrasound time (40 min)

To 100-500 bp fragments. ② Compared with scientz 18-A (Figure S1), scientz 08-III is between different sample volumes

(50-300 μL) has a consistent interrupting effect, indicating that the equipment has good stability. Therefore, the experimental results of this group show that through

One step to optimize the length of ultrasound, scientz 08-III can interrupt the chromatin to 100-250 bp (mononucleosome size), and the device is stable

Well, it may also have good expressive power in a system with a volume greater than 300 μL, and the upper limit of the volume still needs further testing. The device

There is a lot of room for improvement, and it is entirely possible to upgrade to an excellent DNA interrupter for ChIP experiments. In order to demonstrate these performance and potential,

More testing experiments are needed (some of which have been discussed orally with the road manager).