What should I do if the gene electroporator bursts?

FAQ: Gene Electroporator

  1. How to judge the popping cup?

  2. After the explosion, can the electric cup be used?

  3. After the explosion, can the transformation be successful?

  4. Why is the explosion?

  5. How to avoid it?

1 Simple, as the name suggests, you can hear the embarrassment flashing in the cup.

2 After the explosion of the cup, it can often be seen that the electrode of the cup has become black, and even the cup has cracks. It is generally recommended not to use (electrode cups are all disposables in foreign countries). However, what I know is not a one-time domestic one, it is all reused.

3 After the explosion, the conversion efficiency is significantly reduced, of course, more or less.

4 The reason is that there are too many ions in your system. If it is the conversion of the linked product, it must be precipitated, dissolved and then converted, and dissolved in water instead of TE. If it is a plasmid, make sure it has been thoroughly washed with 70% ethanol at the time of extraction.

5 After knowing the reason, just follow the above, and you should not touch the electrode of the electric rotor.