Principle of PCR instrument technology

The Taqman probe labeled with fluorescein is mixed with the template DNA to complete the thermal cycle of high temperature denaturation, low temperature renaturation, moderate temperature extension, and the polymerase chain reaction rule is followed. The Taqman probe complementary to the template DNA is cleaved and fluorescent. The pigment is free in the reaction system and emits fluorescence under specific photoexcitation. As the number of cycles increases, the amplified target gene fragment grows exponentially, and the fluorescence signal intensity changes with the amplification as measured by real-time detection. The CT value is obtained, and at the same time, using a plurality of standards of known template concentrations as a control, the copy number of the gene of the specimen to be tested can be obtained.