SCIENTZ-150 High-Pressure Homogenizer: Breaking Through The Final Barrier Of Lactobacillus Cell Disruption

Anyone working with Lactobacillus will eventually face the same question:

Have the cells really been disrupted?

The dense peptidoglycan cell wall of Lactobacillus makes the extraction of intracellular proteins, polysaccharides, and postbiotics particularly challenging. Traditional methods such as ultrasonication and bead milling each have limitations, including localized overheating, scale-up difficulties, and contamination risks. High-pressure homogenization has emerged as a cleaner, more reliable solution for efficient cell disruption while preserving biological activity.

01

Up to 1500 Bar for Efficient Cell Disruption

SCIENTZ-150

The SCIENTZ-150 Experimental High-Pressure Homogenizer utilizes a precision homogenizing valve system. Materials are pressurized by ceramic plungers and forced through micron-scale gaps, generating intense shear forces, cavitation effects, and collision impacts. These mechanisms work together to effectively rupture tough bacterial cell walls, achieving a disruption rate greater than 95% for Lactobacillus strains.

02

Ideal for Small-Volume R&D Applications

Early-stage strain development often involves valuable samples available only in limited quantities. The SCIENTZ-150 is optimized for low sample volumes, enabling researchers to complete disruption, extraction, and analysis workflows with minimal material consumption. Process parameters can be validated cost-effectively and then seamlessly scaled using the system's variable-frequency flow control range of 6–12 L/h.

03

Integrated Temperature Control as a Standard Feature

Temperature rise is inevitable during high-pressure processing, yet many target biomolecules are heat-sensitive. The SCIENTZ-150 incorporates a patented cooling structure that provides continuous temperature control throughout homogenization. When connected to a thermostatic cooling system, sample temperatures can be maintained between 10–15°C, helping preserve protein integrity, polysaccharide quality, and overall biological activity.

04

Streamlined Operating Workflow

The recommended workflow includes pre-cooling, system cleaning, homogenization at 900–1200 bar for three cycles, sample collection and evaluation, followed by cleaning and storage procedures. The system supports feed materials with particle sizes ≤500 μm, viscosities ≤2000 cP, and temperatures ≤90°C.

Designed for Clean, Scalable Processing

Unlike bead milling, the SCIENTZ-150 introduces no grinding media, reducing contamination risks and eliminating consumable replacement requirements. An integrated degassing function and complete drainage valve structure help prevent cross-contamination between batches. All wetted parts are manufactured from 316L stainless steel and comply with food and pharmaceutical hygiene standards.

Conclusion

From method development using precious small-volume samples to stable pilot-scale production, the SCIENTZ-150 addresses more than just whether cells can be disrupted. It delivers a scalable, reproducible, and contamination-free solution that enables researchers and manufacturers to achieve consistent results with confidence.