High-pressure Homogenizer: A Full-process Solution For Nano-dispersion From Laboratory To Mass Production

High-pressure homogenization technology has become the core process for mRNA vaccine liposome preparation, nanomaterial dispersion and other high-end fields due to its nano-scale particle size control technology and excellent stability. Through multiple action mechanisms such as high-pressure shearing and cavitation effect, it has demonstrated significant advantages in cross-fields such as biomedicine, food engineering, and new energy, and is particularly suitable for industrial continuous production needs. As an industry technology leader, SCIENTZ Bio has built a full-process solution from laboratory research to industrial production. Relying on its independently innovative core technology and a complete equipment system, its products have been widely used in well-known domestic and foreign pharmaceutical companies, biotechnology companies and top scientific research institutes, effectively helping customers break through process bottlenecks and accelerate the transformation and industrialization of scientific research results.

1. Comparison of Common Methods

High-pressure homogenization: High-pressure homogenization materials pass through a narrow homogenization valve (micrometer-level gap) under high pressure, and experience high-speed shear, cavitation effect, turbulence and collision, so that particles/droplets are broken to the nanometer level.

Ultrasonic crushing: Ultrasonic crushing uses ultrasonic probes to generate high-frequency vibrations to form cavitation bubbles in the liquid. When the bubbles implode instantly, local high temperature, high pressure and microjets will be generated, thereby crushing particles or dispersing agglomerates.

High-speed shearing: High-speed shearing relies on surfactants to reduce interfacial tension, and mechanical stirring is used to form an emulsion of immiscible liquid phases.

Technical Method	Advantages	Disadvantages
High - pressure homogenization	- Uniform particle size (can reach nanometer level) - Efficient continuous production, suitable for industrialization - Wide applicability (liquid/emulsion/suspension)	- Relatively high equipment cost - Limited in processing high - viscosity materials
Ultrasonic treatment	- Simple operation, suitable for small - scale experiments - No need for complex pretreatment	- Low efficiency, difficult to scale up - Prone to heat generation, may damage heat - sensitive components - Probe is easily worn
High - speed shearing	- No need for expensive equipment - Suitable for scenarios with low - precision requirements	- Dependent on surfactants, may affect product purity - Complex post - treatment, need to remove residual reagents - Difficult to achieve nanometer - level dispersion

Summary: High-pressure homogenization can meet the needs of customers with relatively high particle size requirements. At the same time, it can take into account both experimental and production needs and has a wide range of applications.

2. Some cases of SCIENTZ

Food industry

Equipment: SCIENTZ-150, constant temperature bath

Sample: 200-500 mL raw milk

Parameters: Pressure: 20 MPa pressure condition, three homogenization cycles, temperature 50 degrees

Results: After raw milk homogenization, the particle size of MFG was reduced by 160%, from 557 to about 220 nm;

Figure 1. Changes in MFG particle size and zeta potential during gastrointestinal digestion

Figure 2 Particle size analysis of homogenized milk

Biomedicine

Equipment: SCIENTZ-207A, constant temperature bath

Sample: 200 mL Escherichia coli

Parameters: Pressure: 90-100 MPa, time: 10 min, cooling temperature: 8°C

Results: After homogenization, the bacterial solution was fully broken and the inclusion bodies were released efficiently

Equipment: SCIENTZ-207A

Sample: Chitosan

Results: The viscosity of the reverse PAPI emulsion with chitosan nanoparticles was effectively enhanced compared with the emulsion without stabilizer.

API/ChNF-L, PAPI/ChNF-S, PAPI/ChNC Shear viscosity, (g-i). Comparison of initial viscosity with PAPI

Figure 1. Viscosity of reversed polymethylene polyphenyl polyisocyanate (PAPI) emulsion

(a). Dry and wet strength of plywood; (b). Change of bonding strength with storage time;

Figure 2 Shear strength of plywood

Application of high pressure homogenizer

Bioengineering

(1) Processing of microbial samples and disruption of algae cells;

(2) Research on the functional effects of plant proteins

Food engineering

(1) Effects on the physical and chemical properties of food and beverages;

(2) Application of non-heating sterilization technology in liquid foods

Biomedicine

(1) Research on the extraction and preparation process of drug ingredients;

(2) Research on drug delivery mechanism

Chemicals and new materials

Uniform dispersion of nanoparticles such as graphite, carbon nanotubes, and silica; (2) High-stability emulsification of dyes, pigments, and coatings

Daily chemicals and cosmetics

(1) Optimize the microemulsion system of detergents to improve the cleaning ability;

(2) Nanocarrier packaging of essences and sunscreens (enhance permeability)

New energy and batteries

Uniformly distribute lithium battery electrode materials (such as positive and negative electrode slurries); (2) Optimize the dispersibility of fuel catalyst slurries

The key process to unlock nano-level precision is to choose high-pressure homogenization technology. SCIENTZ Biotech specializes in providing high-pressure homogenization full-process solutions from laboratory research to industrial mass production. With the core advantages of the SCIENTZ series of equipment (such as the efficient and stable 207A, etc.), we help customers achieve excellent nano-dispersion effects and process optimization in the fields of mRNA vaccines, nanomaterials, biomedicine, food engineering, etc., and accelerate the transformation of R&D results.