Steps for preparation of microprojectiles for gene guns

Gold powder mother liquor configuration

Weigh 60mg of gold powder into Eppendorf

1 ml of 75% ethanol was added, vortexed thoroughly, allowed to stand for 15 min, and centrifuged at 1500 rpm for 5 min.

The supernatant was discarded, 1 ml of sterile water was added, vortexed thoroughly, and centrifuged at 1500 rpm for 5 min. Repeat 3 times

Discard the supernatant, add 1 ml of sterile 50% glycerol, vortex well, store at -20 ° C