Application of gene gun in transgenic plants

Steps to grow genetically modified plants using a gene gun

1. The gene gun sends the exogenous DNA wrapped in gold powder or tungsten powder directly into the complete plant tissues and cells through high-pressure helium high-speed microprojectiles.

   
   

2. Plant regeneration through cell and tissue culture techniques

   
   

3. Transgenic plants are selected as transgenic plants

Advantages of using genetic guns to make genetically modified plants

1. Not limited by recipient plant range

2. The construction of the vector plasmid is relatively simple

At present, the gene gun method is a widely used method in the research of genetic modification.