CRY-3B Laboratory Electric Cell Fusion System

Name: CRY-3B Laboratory Electric Cell Fusion System Factory and Suppliers China - Customized Products Wholesale - Scientz Biotechnology
Brand: Scientz
SKU: 50626640

CRY － 3B Syncretize Electric suitable for cell hybridization and cell fusion and direct observations can be made under inverted microscopes. It has a widespread application in the research on microorganism, animal medicine and bio-engineering. It provides safety, ease of use, and efficiency as...

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Description

Technical Parameters

Product Overview

The CRY-3B Integrated Electric Cell Fusion Instrument is suitable for cell hybridization and cell fusion experiments, enabling direct observation under an inverted microscope. It is widely applied in microbiology research, veterinary medicine, and bioengineering fields. Compared with conventional methods, it offers enhanced safety, ease of use, and efficiency. Both planar electrodes and needle electrodes are available for selection.

Core Performance Features

Dual Functions: Capable of both cell electrofusion and electroporation.

Wide Parameter Range: Flexible adjustment of voltage and pulse time to meet diverse experimental needs.

Fast & Efficient: Completes cell arrangement, fusion, and post-processing in just a few seconds; simple and rapid post-operation steps.

Intuitive Operation: LCD touch screen for easy and quick data setting and operation.

Applications

Cell fusion (fusion process observable under inverted microscope)

Nuclear transfer

Embryo manipulation

Hybridoma production

Plant protoplast fusion

Chicken embryo gene delivery

Live cell gene/drug transfection

Main Technical Parameters

Parameter	Specification
AC Electric Field Parameters
Bunch Pulse Voltage	Peak-peak value: 0-58V (0-±29V), continuously adjustable
Bunch Pulse Frequency	30KHz-3000KHz, adjustable
Gate Time	10-1000μs, step adjustment of 1μs, continuously adjustable
DC Electric Field Parameters
Square Wave Fusion Pulse Voltage	5-600V adjustable (based on electrode distance); electric field intensity up to 6000V/cm at 1.0mm electrode distance
Square Wave Fusion Pulse Duration	5-5000μs, step adjustment of 1μs, continuously adjustable
Number of Fusion (Square Wave) Pulses	1-9 pulses
Standard Electrode	Fusion planar electrode with 0.5mm polar distance (optional electrodes listed below)
Net Weight	9.22Kg
Packaging Size	450×370×290mm

Optional Microscope Slide Electrodes

Model	Electrode Distance	Volume	Voltage Range
1－0.5	0.5mm	20µL	0-600V DC
1－1.0	1.0mm	40µL	0-600V DC
1－2.0	2.0mm	80µL	0-600V DC
1－3.2	3.2mm	700µL	0-600V DC
1-10	10mm	2200µL	0-600V DC

Maintenance of Optional Electrodes

Clean with mild detergent, ethanol, or sterilize via ethylene oxide.

Cell Fusion Induction Methods

There are three main methods for inducing cell fusion:

Biological Methods (Virus-mediated): Viruses such as Sendai virus, parainfluenza virus, and Newcastle disease virus possess fusion proteins that mediate fusion between viruses and host cells, as well as cell-to-cell fusion. Live viruses can be used to induce cell fusion.

Chemical Methods (PEG-mediated): Polyethylene glycol (PEG) induces changes in the arrangement of membrane lipid molecules. After removing PEG, the plasma membrane restores its ordered structure, and adjacent cells can fuse during this recovery process.

Physical Methods (Electrical/laser-mediated): Electrical excitation or laser treatment disrupts the plasma membrane temporarily, allowing cell fusion when the membrane repairs.

Application Value of Cell Fusion Technology

Cell fusion technology is not only widely used in basic research but also holds significant practical value:

Plant Breeding: Successful cultivation of hybrid crops such as radish-cabbage, Nicotiana glauca × Nicotiana langsdorffii, and tomato-potato.

Agriculture, Industry, and Medicine: Achieved groundbreaking research results, with continuously expanding application fields.

Animal Somatic Hybridization:

Gene mapping: Correlates the presence/absence of chromosomes or fragments in hybrid cells with trait expression, enabling gene localization. For example, Weise and Green (1967) discovered preferential loss of human chromosomes in human-mouse hybrid cells, facilitating gene mapping on human chromosomes. Ruddle et al. (1970) systematically used fusion cells for human gene mapping.