Cell Disruption Ultrasonic Homogenizer For Lab

Process for preparing protein samples using Cell disruption Ultrasonic Homogenizer：

The protein sample preparation process is simply three steps: breaking, precipitating proteins and removing impurities.

There are many ways to break. Let me introduce the advantages of using the Cell disruption Ultrasonic Processor for ultrasonic fracture.

Ultrasonic crushing:

1. Minimize proteolysis and inhibit protease degradation.

2. Reduce the effects of operating process temperatures or other physicochemical conditions on protein denaturation.

3. Reduce the contamination of nucleic acid contamination by subsequent electrophoresis and other analysis.

Ultrasonic cell crusher principle：

The input of high-energy ultrasonic waves can break the cells, and the mechanism may be related to the cavitation phenomenon of bubble generation, growth and fragmentation when a strong acoustic wave acts on the solution. Shock waves and shear forces caused by cavitation cause cells to lyse. The efficiency of sonication depends on the frequency, sound energy, processing time, cell concentration, and cell type.

Experimental steps:

1. Prepare the cell suspension, buffer, Ultrasonic cell Processor

2. The washed tissue is chopped into small pieces with a stirrer, suspended in at least twice the volume of the buffer; the cells of the culture solution are washed away (the bacteria are suspended in at least two volumes of the buffer);

 3. The ultrasonic probe is immersed in the suspension for sonication. Generally, the total ultrasonic time is divided into several cycles, such as 4 × 30 s, and the sample is allowed to cool in an ice bath between cycles.

Precautions

1. Ultrasonic disruption is commonly used in a variety of bacterial and brain tissue homogenates. According to the specific circumstances, the crushing time can be appropriately extended, generally not more than 10 minutes.

2. Check the efficiency of cell disruption with phase contrast microscopy.

3. One point to note about ultrasonic disruption is that the probe should remain below the surface of the suspension throughout the process.

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