Gene Electroporator

Product Description
The Gene Electroporator consists of the main unit, gene electroporation cup, and dedic ated connecting cables. lt pri marilyuses electro poration to introduce DNA into competent cels,plant and animal cells, and veast cells, Compared to other methods, the peneelectroporator oflers high repeata bility, high eficiency, ease of operation, and prec ise control.Addition all,electroporation has no &enotoxicitymaking it an essential technigue in molecular biology.
Working Principle
Electroporatio n, also known as cellular elect roporation,is a key metho d for intro ducing exogenous macromolecules like DNA, RNA, SiRNA and proteins, as well as small molecules, into celks.Under the influence ofa transient strong electric field, thecel membrane becomes permeable, allowingcharged exogenous substances toenter the cellin a manner similar to electro phoresis. Dueto the high resistance of thecellmembrane's phospholipid bilayer,the voltage acrosthe cell is mostly bore by the membrane, with minimal voltagein thecyto plasm. This leads to negligible cytotoxicity during electro porationunder normal conditions, Once bNA or other substances pass through the membrane, they stay nearthe membrane before the celf' s m echanisms transport them to the nucleus or other parts of the cell.
Since electroporation relies on physical methods, the molecular characterist ics on the cell surface have minimal efiect on the process. U nlikechemical transfection orvi ral vector methods, electro poration can be applied to all cell types and is easily quantifilable

Application

FoodAgricultural Veterinary BioengineeringIndustry
·Electroporation ofbacteria,yeast,and other microorganisms.

·Transfection ofmammalian celb,plant tisues,and protoplasts.

·Cell hybridization and gene fusion.

·Inroduction of markergenes for labeling and indicating purpos6.

·Inroduction of drugs,proteins,antibodies,and other molecules
for studying cellular structureand function.

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