Electroporator Manufacturer

Electroporation, also known as cellular electroporation, is a key method for introducing exogenous macromolecules like DNA, RNA, siRNA, and proteins, as well as small molecules, into cells.
Under the influence of a transient strong electric field, the cell membrane becomes permeable, allowing charged exogenous substances to
enter the cell in a manner similar to electrophoresis. Due to the high resistance of the cell membrane's phospholipid bilayer, the voltage across
the cell is mostly borne by the membrane, with minimal voltage in the cytoplasm. This leads to negligible cytotoxicity during electroporation
under normal conditions. Once DNA or other substances pass through the membrane, they stay near the membrane before the cell's mecha
nisms transport them to the nucleus or other parts of the cell.
Since electroporation relies on physical methods, the molecular characteristics on the cell surface have minimal effect on the process. Unlike
chemical transfection or viral vector methods, electroporation can be applied to all cell types and is easily quantifiable.