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Ultrasonic Homogenizer Nano Graphene Machine Ultrasonic Dispersing

Ultrasonic Homogenizer Nano Graphene Machine Ultrasonic Dispersing

Basic principle of crushing From the definition of pulverization, it is known that the feed pulverization uses the pulverizing tool (the hammer, the sieve, the tooth plate of the hammer mill, the pressure roller of the roller mill, the steel ball of the ball mill, etc.) to apply force to the...

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Basic principle of crushing


     From the definition of pulverization, it is known that the feed pulverization uses the pulverizing tool (the hammer, the sieve, the tooth plate of the hammer mill, the pressure roller of the roller mill, the steel ball of the ball mill, etc.) to apply force to the material when the effect exceeds the material. The cohesive force (binding force) between the particles is sometimes broken. As the pulverization process progresses, the specific surface area of the material continuously increases, and the solid feed breaks into small pieces or the number of fine powders increases. This process is generally just a change in geometry.


Material crushing method



1. Shearing This is a low-energy pulverizing method that can pulverize tough materials. The newly formed surface is relatively regular and easy to control the size of the grain. Generally, dicing, slicing, shredding, fruit, vegetable, meat, Didics fall into this category. Shearing also plays an important role in the Rabbi-to-roll mill for the skin grinding system of wheat milled flour.


2. Impact When the material and the working member collide at a relatively high speed, the material is crushed by a very short time variable load. Brittle materials for mass snares are particularly suitable for this type of comminution. The degree of pulverization of the impact pulverization is large, and it can be used from the crushing of a large block to the fine pulverization, and it is possible to pulverize various materials. The most typical is a hammer mill, which is used in the food industry. There is also a machine that uses its own high-speed relative motion to collide and crush, called a supersonic jet mill, but it consumes a lot of energy.


3. Physical law

A method of disrupting tissue cells mainly by various physical factors. Common methods used in biochemical preparation are:


3.1 repeated freeze-dissolved method


Principle: The cells are destroyed by sudden freezing, the formation of intracellular ice crystals and sudden changes in the concentration of intracellular and extracellular solvents.


Method: The cells to be broken are frozen under -20 degrees, melted at room temperature, and repeated several times. The cell structure is broken due to the formation of ice particles in the cells and the increase of the salt concentration of the remaining cell liquid.


Features: This method is suitable for tissue cells, mostly used for animal materials, and has a poor effect on microbial cells.


3.2 Rapid thermal quenching method Put the material into boiling water for 85-90 minutes and cool it rapidly in the water bath. This method can be used for bacteria and virus materials.


3.3 Ultrasonic treatment The cell suspension is treated with a certain amount of ultrasonic wave to make the cells violently oscillate and rupture. This method is more suitable for microbial materials. Escherichia coli is used to prepare various enzymes. The concentration of 50-100 mg of bacteria/ml is often used. Ultrasonic waves of 15 to 20 KHz can perform cell disruption under high-intensity input of sound energy. Its fracture mechanism: may be related to shock waves and shear forces caused by cavitation. The efficiency of sonication is related to factors such as audio, sound energy, processing time, cell concentration and the first type.


Features: simple operation, good repeatability, saving time; mostly used for microbial and tissue cell disruption. There are problems: sensitive to ultrasound and nucleic acids should be used with caution. Cavitation is the direct cause of cell destruction and produces reactive oxygen species, so some sulfhydryl protecting agents are added.

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