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The Application Case Of The Gene Gun
Jul 28, 2018

User: School of animal science and technology, Shanxi Agricultural University, 2015

Uses: transfer the pwPICZalpha-REG3a gene to the Pichia yeast strain X33, and transfer the pwPICZalpha recombinant plasmid with XhoI-porcine-NK-lysin-EcoRI to the Pichia yeast strain X33

Model: new organism, unknown

Parameter: unknown

Number: JYQ001

 

User: College of life science, Southwestern University, 2014

Purpose: to import GFP- PtoWRKY60 vector into onion epidermal cells.

Model: GJ-1000

Condition: unknown

Number: JYQ002

 

User: School of Animal Medicine, China Agricultural University, 2012

Purpose: bombardment of Eimeria maxima of non sporocyst oocysts with plasmid DNA encoded yellow granules of enhanced yellow fluorescent protein (EYFP).

Model: GJ-1000

Conditions: 1.6 mm m tungsten particles, 7 Mpa (1015 psi) helium pressure, 65 mm target distance and -0.098 Mpa (24.8 '' Hg) chamber vacuum.

Number: JYQ003

 

User: College of life science, Southwestern University, 2015

Purpose: to import GFP- PtoWRKY60 vector into onion epidermal cells.

Model: GJ-1000

Condition: unknown

Number: JYQ004

 

Users: Shanghai Jiao Tong University in 2010

Purpose: To introduce the target gene into soybean in vitro growth site

Model: GJ-1000

Condition: the diameter of the target DNA is 1.5 micron m gold particles: 60 micron g Au powder coated with 1 g DNA

Number: JYQ005

 

User: Institute of Oceanography, Chinese Academy of Sciences, 2012

Purpose: to import plasmid vector p35bar40tac carrying bar gene into the gametophyte of the Chinese cabbage.

Model: GJ-1000

Conditions: bombardment pressure 7 MP, vacuum degree -0.085 MP, bombardment distance 6 cm

Number: JYQ006